Tag

Part:BBa_K4766005:Design

Designed by: Kaiyang Lv   Group: iGEM23_DUT-China   (2023-10-05)


DogTag


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 19
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 19
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 19
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 19
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 19
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

DogTag can be attached to a protein so that it can be captured by proteins connected with DogCatcher.

Source

References

Keeble AH, Yadav VK, Ferla MP, Bauer CC, Chuntharpursat-Bon E, Huang J, Bon RS, Howarth M. DogCatcher allows loop-friendly protein-protein ligation. Cell Chem Biol. 2022 Feb 17;29(2):339-350.e10.